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KMID : 0378120030300020127
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Chungnam Medical Journal
2003 Volume.30 No. 2 p.127 ~ p.136
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Analysis of Glycoproteins from Toxoplasma gondii by Two Dimensitional Gel Electrophoresis
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Hwang Il-Young
Choi In-Wook
Shin Dae-Whan
Lee Young-Ha
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Abstract
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Serious disease due to Toxoplasma gondii occurs in congenitally infected children and in immunosuppressed patients, particularly in the case of HIV infection. Glycoproteins modulate the physicochemical properties, and are involved in biological and immunological activities. Although there are many reports about the immune responses after T. gondii infection, there are few reports to identify the glycoproteins from T. gondii. For this reason, we purified the glycoproteins from tachyzoites and cysts of T. gondi and then evaluated the antigenic characterizations of toxoplasmic glycoproteins.
Tachyzoites of T. gonii were purified by Percoll-gradient centrifugation. Glucosamine was the major monosaccharides released from the tachyzoites of T. gondii by acid hydrolysis methods. In order to define the antigenicities of both strains, we did 2-dimensional electrophoresis(2-DE). Over 40 protein spots were reproducibly separated in tachyzoites of T. gondii by 2-DE in range of the pH 5-8. Glycoproteins were isolated from tachyzoites of T gondii by Con A-Sepharose 4B affinity chromatography. The major bands of glycoproteins were approximately 22 kDa, 30 kDa and 38 kDa in tachyzoites of T. gondii
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KEYWORD
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Toxoplasma gondii, Glycoprotein
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